IRI - Institut de Recherche Interdisciplinaire

2012

2012-05-16T14:57:01Z

A Rab11A/Myosin Vb/Rab11-FIP2 Complex Frames Two Late Recycling Steps of Langerin from the ERC to the Plasma Membrane
A. Gidon, S. Bardin, B. Cinquin, J. Boulanger, F. Waharte, L. Heliot, H. de la Salle, D. Hanau, C. Kervrann, B. Goud, J. Salamero
Traffic, 13:815-833 (2012).

Séminaire d'Isabelle Landrieu le 12 juin 2012 à 10h00 à l'IRI

guillaume — 2012-05-11T12:23:34Z

Modulation of protein-protein interactions by the phosphorylation code of the neuronal protein Tau

Isabelle Landrieu
UMR8576 CNRS, North of France University
Interdisciplinary Research Institute, Hôtel à Projets
Villeneuve d'Ascq, France

We are focusing on the deciphering of the complex phosphorylation code of Tau and its implication in Alzheimer Disease. We combine in vitro enzymatic reactions with recombinant kinases to phosphorylate the neuronal Tau protein, and Nuclear Magnetic Resonance spectroscopy to unravel the resulting phosphorylation pattern. Beyond the analytical capacity, this approach is followed by functional assays with the same samples to characterize protein-protein interactions (PPI) depending on specific phosphorylation patterns of Tau. We have shown for example that Tau interaction with ApoE is specifically dependent on prior phosphorylation by GSK3beta or that interaction with 14-3-3 depends on PKA phosphorylation. Some of these phospho-specific PPI will in turn regulate further post-transcriptional modifications of Tau, such as the interaction with the phospho-dependent prolyl cis/trans isomerase Pin1 that modulates Tau dephosphorylation by the PP2A phosphatase. In addition to deciphering the complex biology of Tau, this methodology can also be used to characterize the phospho-epitopes recognized by monoclonal antibodies.


Isabelle Landrieu 2012/06/12: UMR8576 - IRI

97200. Séminaire d'Arnaud Legrand le 13 mars 2012 à l'IRI

guillaume — 2012-02-24T08:26:05Z

Study of the interaction between the oncoprotein Ets-1 and the enzyme PARP-1 : A new therapeutic approach in cancer ?

Arnaud Legrand
Institut de Recherche Interdisciplinaire

Ets-1 is a transcription factor of the Ets family. When overexpressed, it is involved in the development of invasive pathologies such as rheumatoid arthritis and cancers. Ets-1 regulates gene expression with partners by binding to specific DNA elements, called Ets binding sites (EBS), found in the promoters of its target genes. Therefore, the understanding of the specificity of its functions is linked to the characterization of its partners. For this purpose, we used an affinity purification strategy of Ets-1 partners using biotinylated Ets-1 for streptavidin pull-down. Several potential interaction partners were identified by MALDI-TOF mass spectrometry. Among those, we were able to identified, interacting with Ets-1, the Poly (ADP-Ribose) Polymerase-1 (PARP-1). PARP-1 is an abundant nuclear protein which catalyzes poly-ADP-ribosylation (PARylation) and plays diverse roles in many molecular and cellular processes, including DNA damage detection and repair and chromatin modification. Previous studies have revealed that besides its role in DNA repair pathway, PARP-1 is involved in the regulation of different transcription factors. In this study, we show that Ets-1 interacts directly with PARP-1. Using PARP-1 catalytic inhibitors, our results show that parylation of Ets-1 has direct consequences on its activity. Taken together, these findings strengthen the idea of a functional link between transcription factors and DNA repair proteins. Furthermore, inhibition of PARP-1 could be a new strategy to target Ets-1 activity in tumours.

2012

rabah — 2012-01-27T12:25:06Z

1. Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small biomolecules
Yannick Coffinier, Hervé Drobecq, Sabine Szunerits, Oleg Melnyk and Rabah Boukherroub
Nanoscale 4 (2012) 231-238

2. Thiol-yne reaction on boron-doped diamond electrodes
Dalila Meziane, Alexandre Barras, Alexander Kromka, Rabah Boukherroub and Sabine Szunerits
Anal. Chem. 84 (2012) 194-200

3. Preparation of Graphene/Tetrathiafulvalene nanocomposite switchable surfaces
Izabela Kamińska, Manash R. Das, Yannick Coffinier, Joanna Niedziółka-Jönsson, Patrice Woisel, Marcin Opałło, Sabine Szunerits and Rabah Boukherroub
Chem. Commun. 48 (2012) 1221-1223

4. Sliding droplets on superomniphobic zinc oxide nanostructures
Guillaume Perry, Yannick Coffinier, Vincent Thomy, and Rabah Boukherroub
Langmuir 28 (2012) 389-395

5. Hydrogen peroxide sensor based on horseradish peroxidase immobilized on aminated diamond interfaces
Qi Wang, Alexander Kromka, Jana Houdkova, Oleg Babchenko, Bohuslav Rezek, Musen Li, Rabah Boukherroub, and Sabine Szunerits
Langmuir 28 (2012) 587-592

6. Dielectric coated plasmonic interfaces : their interest for sensitive sensing of analyte-ligand interactions
Sabine Szunerits, Atef Shalabney, Rabah Boukherroub and Ibrahim Abdulhalim
Reviews in Analytical Chemistry (in press)

7. Convenient and efficient one-pot method for the synthesis of 2-amino-tetrahydro-4H- chromenes and 2-amino-4H-benzo[h]chromenes using catalytic amount of amino-functionalized MCM-41 in aqueous media
M. Mirza-Aghayan, S. Nazmdeh, R. Boukherroub, M. Rahimifard, A. A. Tarlani, M. Abolghasemi-Malakshah
Synthetic Communications (accepted)

8. Wetting parameters optimization for simple and low-cost fabrication of PDMS microfluidic round channels
Magalie de Ville, Philippe Coquet, Philippe Brunet and Rabah Boukherroub
Microfluidics and Nanofluidics (in press)

9. Laser Desorption Ionization Mass Spectrometry of Protein Tryptic Digests On Nanostructured Silicon Plates
Mathieu Dupré, Yannick Coffinier, Rabah Boukherroub, Sonia Cantel, Jean Martinez and Christine Enjalbal
J. Proteomics (accepted)

10. Zipping effect on omniphobic surfaces for controlled deposition of minute amounts of fluid or colloids
Renaud Dufour, Philippe Brunet, Maxime Harnois, Vincent Thomy, Rabah Boukherroub and Vincent Senez
Small (accepted)

11. From micro to nano re-entrant structures : Hysteresis on superomniphobic surfaces
G. Perry, R. Dufour, M. Harnois, Y. Coffinier, V. Thomy, V. Senez, R. Boukherroub
Journal of Adhesion Science and Technology (accepted)

12. One-step reduction and functionalization of graphene oxide sheets using biomimetic dopamine derivatives
Izabela Kamińska, Manash R. Das, Joanna Niedziółka-Jönsson, Patrice Woisel, Joël Lyskawa, Marcin Opałło, Rabah Boukherroub and Sabine Szunerits
ACS Applied Materials & Interfaces (accepted)

13. Investigation of the corrosion behaviour of steel coated with amorphous silicon carbon alloys
Chahrazad Saadi, François Ozanam, Yannick Coffinier, Christoph Gabler, Josef Brenner, Rabah Boukherroub, Mohamed Salah Medjram, and Sabine Szunerits
Surface and Coatings Technology (accepted)

14. Inhibiting proteins biofouling using graphene oxide in droplet-based microfluidic microsystems
Guillaume Perry, Vincent Thomy, Manash R. Das, Yannick Coffinier and Rabah Boukherroub
Lab Chip (accepted)

Membres

philippe — 2012-01-20T09:27:36Z

Philippe Heinrich - 20. Membres

Publications 2012

guillaume — 2012-01-06T09:21:18Z

Ralf Blossey, Jean-François Bodart, Anne Devys, Thierry Goudon and Pauline Lafitte
Signal propagation of the MAPK cascade in Xenopus oocytes : role of bistability and ultrasensitivity for a mixed problem
Journal of Mathematical Biology 64, 1-39 (2012)
DOI : 10.1007/s00285-011-0403-y (03/02/2011)

A Dynamical Model of Oocyte Maturation Unveils precisely orchestrated meiotic decisions
Benjamin Pfeuty, Jean-Francois Bodart, Ralf Blossey, Marc Lefranc
Plos Computational Biology
Research Article, published 05 Jan (2012)
doi:10.1371/journal.pcbi.1002329

A.M. Bouchet, F. Lairion, J.-M. Ruysschaert and M.F. Lensink
Oligoarginine vectors for intracellular delivery : Role of arginine side-chain orientation in chain length-dependent destabilization of lipid membranes
Chemistry and Physics of Lipids 165 (2012) pp 89-96
http://dx.doi.org/10.1016/j.chemphyslip.2011.11.008

Saswat Mohapatra

Emanuele — 2011-12-19T14:41:57Z

Saswat is currently working on Caulobacter cell cycle. Email : Saswat.Mohapatra [at] iri.univ-lille1.fr - Saswat Mohapatra, Postdoc

Francesco Pini, Postdoc

Emanuele — 2011-12-19T14:38:13Z

Francesco is currently working on S. meliloti cell cycle. Email : francesco.pini [at] iri.univ-lille1.fr - Francesco Pini, Postdoc

Nouvel article

vincent — 2011-11-29T13:26:40Z

2010

Delattre AS, Clantin B, Saint N, Locht C, Villeret V, Jacob-Dubuisson F.

Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily. FEBS Journal 2010 ; 277 (22) : 4755-4765.

Herrou J, Bompard C, Wintjens R, Dupré E, Willery E, Villeret V, Locht C, Antoine R, Jacob-Dubuisson F.

Periplasmic domain of the sensor-kinase BvgS reveals a new paradigm for the Venus flytrap mechanism. Proc Natl Acad Sci U S A. 2010 ; 107(40) : 17351-17355.

Willand N, Desroses M, Toto P, Dirié B, Lens Z, Villeret V, Rucktooa P, Locht C, Baulard A, Deprez B.

Exploring Drug Target Flexibility Using in Situ Click Chemistry : Application to a Mycobacterial Transcriptional Regulator. ACS Chem Biol. 2010 Aug 26.

Lens Z, Dewitte F, Monté D, Baert JL, Bompard C, Sénéchal M, Van Lint C, de Launoit Y, Villeret V, Verger A.

Solution structure of the N-terminal transactivation domain of ERM modified by SUMO-1. Biochem Biophys Res Commun. 2010 Aug 13 ; 399(1):104-10.

Clantin B, Leyrat C, Wohlkönig A, Hodak H, Ribeiro Ede A Jr, Martinez N, Baud C, Smet-Nocca C, Villeret V, Jacob-Dubuisson F, Jamin M.

Structure and plasticity of the peptidyl-prolyl isomerase Par27 of Bordetella pertussis revealed by X-ray diffraction and small-angle X-ray scattering. J Struct Biol. 2010 Mar ;169(3):253-65.

Baert JL, Monté D, Verreman K, Degerny C, Coutte L, de Launoit Y.

The E3 ubiquitin ligase complex component COP1 regulates PEA3 group member stability and transcriptional activity. Oncogene. 2010 Mar 25 ;29(12):1810-20.

Dessein AF, Stechly L, Jonckheere N, Dumont P, Monté D, Leteurtre E, Truant S, Pruvot FR, Figeac M, Hebbar M, Lecellier CH, Lesuffleur T, Dessein R, Grard G, Dejonghe MJ, de Launoit Y, Furuichi Y, Prévost G, Porchet N, Gespach C, Huet G.

Autocrine induction of invasive and metastatic phenotypes by the MIF-CXCR4 axis in drug-resistant human colon cancer cells. Cancer Res. 2010 Jun 1 ;70(11):4644-54.

Remise des prix GEFLUC le 2 décembre à 14h00 à l'IRI - Franck Riquet lauréat

guillaume — 2011-11-25T10:34:55Z

La remise des prix GEFLUC (Groupement des Entreprises Françaises dans la Lutte contre le Cancer : http://www.gefluc.org/) aura lieu le vendredi 2 décembre à 14h à l'institut de Recherche Interdisciplinaire (IRI USR 3078)

Le Dr. Franck Riquet, de l'Université Lille1 (EA44 79 Laboratoire de Régulation des Signaux de Division), est lauréat du prix GEFLUC (2011-2012), pour le projet intitulé « Rôle de l'activité d'ERK et d'AKT dans la balance quiescence // prolifération des cellules souches cancéreuses de mélanome : mesure par biosenseur rapporteur d'activité kinasique de type KAR ». Ce projet s'intéresse, via une approche originale de senseurs d'activité de type kinase, à l'implication des voies de signalisation majeures contrôlant la ré-entrée dans le cycle cellulaire des cellules souches du mélanome.


GEFLUC 2011-2012: 2011/12/02